Skip to main content
. Author manuscript; available in PMC: 2017 May 16.
Published in final edited form as: Nature. 2016 Nov 16;539(7630):588–592. doi: 10.1038/nature20162

Figure ED6. Ago3 incorporates more TE antisense piRNAs in nibbler mutant flies.

Figure ED6

a, b) Scatter plots displaying the strand bias (antisense divided by sense) of piRNA populations in w1118 versus nibbler -/- ovaries. (a) displays piRNAs bound to Piwi, Aubergine, or Ago3, while (b) displays piRNAs from total ovarian RNA.

c) Plotted are Z-scores of 5'/5' ping-pong levels per TE (63 germline dominant TEs) in w1118 versus nibbler-/- ovaries.

d) Grouping of TEs (63 germline dominant TEs) based on -fold change in piRNA levels between Zucchini-depleted and control ovaries (left). Boxplot indicates Aub/Ago3-bound piRNA levels in wild-type ovaries for defined TE groups (Tukey definition; *** p < 0.001 after Wilcoxon rank-sum test).