a) Boxplots (*** p < 0.001 by two-sided t-test) showing the 3' end definition (see Methods) of Ago3-, Aub-, and Piwi-bound piRNAs isolated from ovaries of the indicated genotypes. Soma-enriched Piwi-bound piRNAs (see Methods) are shown in boxes with diagonal lines.
b) Stacked bar plots displaying the nucleotide composition at the 5' end or position 10 of piRNAs bound to Aub-, Ago3-, or Piwi (isolated from ovaries of indicated genotypes). The plots show the composition within 10 equally sized bins, sorted for their 3' end precision index (see Methods).
c) Boxplots (*** p < 0.001 by Wilcoxon rank-sum test) showing the R2 values of the comparison between the 3' end profiles of Ago3- or Aub-bound piRNAs from w1118 ovaries and those of nibbler-/- ovaries (Zucchini only), those of ovaries depleted of Zucchini (Nibbler only), and those of the calculated composite that provides the highest R2 (best fit).