Table 2. Ability of Ht to facilitate [PSI⁺] induction correlates with polyQ aggregation.

	Moderate polyQ expression		High polyQ expression
PolyQ insert	Cells with aggregates*, %	[PSI+] induction†	Cells with aggregates*, %	[PSI+] induction†
None	0	-	0	-
Q25	0	-	0	-
Q47	0	-	0	-
Q72	0	-	1	+
Q103	1	±	10	++

^*The [psi^-][rnq^-] transformed with centromeric (moderate polyQ expression) or multicopy (high polyQ expression) Ht-GFP expressing vectors were grown for 7-14 generations. Fluorescent foci indicated Ht aggregation. Four or more transformants were analyzed for each plasmid, and the fraction of cells with aggregates was estimated from at least 1,000 cells.

^†The above transformants also carried the [PSI⁺]-inducing construct NM-GFP. The ±, +, and ++, levels of growth on -Ade after transient expression of NM-GFP correspond to very weak, weak, and moderate [PSI⁺] induction, respectively. Nine transformants were analyzed for each plasmid combination. The induction seen here is low in comparison with that seen in the presence of a high [RNQ⁺] variant that causes high-level [PSI⁺] induction (44); see Fig. 1A.