Table 3. Newly appearing NM-YFP aggregates are detected in close proximity to preexisting [RNQ+] aggregates.
[PSI+] induction, h | No. of cells examined with NM-YFP aggregates | No. of cells with/without overlapping Rnq-CFP and NM aggregates* |
---|---|---|
2 | 12 | 11/0 |
4.5 | 30 | 12/0 |
6 | 57 | 41/0 |
All cells in which only NM-YFP aggregates but not Rnq-CFP aggregates were detected had no CFP fluorescence, indicative of the loss of the plasmid bearing the Rnq-CFP construct.