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. 2004 Aug 24;101(35):12946–12951. doi: 10.1073/pnas.0404280101

Fig. 5.

Fig. 5.

Interaction of 35-kDa PF20 with MEIG1. (A) Yeast two-hybrid assay with (+Galactose) or without (–Galactose) inducer shows specific binding of 35-kDa PF20 to MEIG1. p53–large T antigen interaction is displayed as a positive control. PF20 (35 kDa) and empty pB42D serve as negative control. (B) Coimmunoprecipitation of MEIG1-GFP and 35-kDa PF20 from lysates of nontransfected CHO cells (control) and cotransfected CHO cells with N- or C-terminal anti-PF20 antibodies. The immunoprecipitated material was subjected to Western blotting with anti-GFP antibody. The lysate of cotransfected cells contains the MEIG1-GFP (37 kDa) and free GFP (26 kDa), but only MEIG1-GFP is detected in the pull-down with the C-terminal antibody. (C) Colocalization of 35-kDa PF20 and MEIG1 in CHO cells. CHO cells were transfected with 35-kDa PF20/pEGFP-N2 (a), MEIG1/DsRed-N1 (b), or both plasmids (c). Phase contrast and fluorescence images are shown, and in c, a merged image (Lower Right) shows colocalization of PF20-GFP and MEIG1-DsRed.