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. 2016 Dec 19;7:619. doi: 10.3389/fimmu.2016.00619

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Copyright © 2016 Drabek, Janssens, de Boer, Rademaker, Kloess, Skehel and Grosveld.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of heavy-chain-only antibodies (HCAbs). (A,B) An example of SDS-PAGE gels run under non-reducing conditions (A) and reducing conditions (B) shows that HCAbs form dimers. (C) HPLC SMART profiles show a single peak for each antibody, the solubility and affinity measurements for selected HCAb clones. Note that 3A8 and 3B10 are IgG3 HCAbs and are of higher molecular weight due to a longer hinge region.