TABLE 1.
Plasmids used in this study
| Plasmid | Description | Reference or source |
|---|---|---|
| pWWW412 | hrpK promoter for constitutive expression in pOS1 | 51 |
| pLM4 | Thermosensitive pET194ts-based vector with aphA3a | 49 |
| pLM5 | IPTG-inducible spac promoter expression in pLM4 | 49 |
| pJK4 | Thermostable variant of pLM5 | 13 |
| pPhrpK-wpaA | wpaA in pWWW412 | This study |
| pPhrpK-wpaB | wpaB in pWWW412 | This study |
| pLM4-ΔbslS | One-kilobase flanking regions of bslS in pLM4 to delete bslS | This study |
| pLM4-ΔbslT | One-kilobase flanking regions of bslT in pLM4 to delete bslT | This study |
| pLM4-ΔwpaA | One-kilobase flanking regions of wpaA in pLM4 to delete wpaA | This study |
| pLM4-wpaB::aad9 | One-kilobase flanking regions of wpaB and aad9b in pLM4 to delete wpaB | This study |
| pwpaA | wpaA in pJK4; pSY194 | This study |
| pwpaAts | wpaA in pLM5; pSY195 | This study |
| pwpaB | wpaB in pJK4; pSY196 | This study |
| pwpaBts | wpaB in pLM5; pSY197 | This study |
a
aphA3 is a kanamycin resistance determinant.
b
aad9 is a spectinomycin resistance determinant.