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. 2016 Dec 13;199(1):e00665-16. doi: 10.1128/JB.00665-16

FIG 3.

FIG 3

Analysis of antibodies and Fab preparations. (A) Anti-CS2 antibodies and Fab fragments are shown. (left) Western blot of purified CS2 fimbriae using anti-CS2 antisera (R1590) indicating a specific band for the major structural subunit, CotA, at approximately 15 kDa. (right) PageBlue staining after SDS-PAGE of purified anti-CS2 antibodies in Tris buffer (lane 2), purified anti-CS2 antibodies in 1× PBS (lane 3), papain-digested anti-CS2 antisera (lane 4), and purified Fab fractions 1 and 2 in 1× PBS (lanes 5 and 6). Anti-CS2 Fab fraction 2 was used in biomechanical assays. (B) Anti-CFA/I antibodies and Fab fragments are shown. (left) Western blot of purified CFA/I fimbriae using anti-CFA/I antisera (R2175) indicating a specific band for the major structural subunit, CfaB, at approximately 15 kDa. (right) Silver staining after SDS-PAGE of purified anti-CFA/I antibodies in Tris buffer (lane 2), purified anti-CFA/I antibodies in 1× PBS (lane 3), papain-digested anti-CFA/I antisera (lane 4), and purified Fab fraction in 1× PBS (lane 5), which was used in biomechanical assays. Lane 1 shows molecular size markers.