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. 2016 Dec 15;83(1):e02462-16. doi: 10.1128/AEM.02462-16

FIG 6.

FIG 6

Final step in the characterization of GCT stereospecific properties. Traces show the 31P NMR resonances due to the GPI formed by the auxiliary enzymes DIPP synthase/phosphatase in cell extracts of Archaeoglobus fulgidus using CDP-glycerol produced by the GCTs examined in this study (indicated on the left-hand side). (a and b) recombinant AQ1368, (c) recombinant AQ185, and (d) GCT activity partially purified from a cell extract of Archaeoglobus fulgidus. The stereospecificity of each GCT for sn-glycerol 3-phosphate and sn-glycerol 1-phosphate was determined from the areas of the resonances attributed to GPI structure I and GPI structure II in the 31P NMR spectra of the final reaction mixtures.