TABLE 1.
Quantification of thiol groups in methanobactin from M. trichosporium OB3b wild-type, ΔmbnAN + pWG101, and ΔmbnAN + pWG102 strains as isolated and following reduction by tris(2-carboxyethyl)phosphine under anaerobic conditions
| M. trichosporium OB3b strain | No. of thiol groups per methanobactina |
|
|---|---|---|
| As isolated | Following reduction | |
| Wild type | 3.0 × 10−4 ± 5.1 × 10−4 | 1.7 ± 0.3 |
| ΔmbnAN + pWG101 | 3.7 × 10−3 ± 3.2 × 10−4 | 2.1 ± 0.1 |
| ΔmbnAN + pWG102 | 2.2 × 10−3 ± 1.1 × 10−4 | 3.1 ± 0.3 |
a
Values are averages ± standard deviations of results from duplicate samples.