FIG 4.

Transcript levels of iac and cat genes from Paraburkholderia phytofirmans cells exposed to indole-3-acetic acid (IAA). Real-time PCR analysis of iac and cat genes in cells grown on fructose, benzoate, or IAA as a sole carbon and energy source. It should be noted that transcript levels of the ribosomal protein S7 (rpS7) and tryptophan dioxygenase (kynA) genes, used as expression controls, remained unchanged under these conditions. Transcript levels were normalized to the average value of the transcript levels in fructose treatment. 16S rRNA was used as a reference gene (internal control). Note different scales. All experiments were performed in three biological replicates. Error bars represent standard deviations. Different letters indicate statistically significant differences between treatments (one-way analysis of variance, P < 0.05; Tukey's test, P < 0.05).