TABLE 2.
Primer pairs used in this study
| Purpose | Forward |
Reverse |
||
|---|---|---|---|---|
| Primer name | Sequence (5′→3′) | Primer name | Sequence (5′→3′) | |
| Inactivation of genes | Mut iacAFw | GCCCAGTTTCTCGACATGAT | Mut iacARv | GTCGTCTGCAACGAACTGGT |
| Mut iacBFw | CGAACAGATCGGCGAAGT | Mut iacBRv | CATAGCGCCACAGGTTGTATT | |
| Mut iacCFw | GGTCA ACGTCTTGCA GAACC | Mut iacCRv | GTTTCGTCGTCGATCGATTT | |
| Mut iacDFw | GACTATCGCGAGTGGCTCA | Mut iacDRv | CTTGCAAAACTGATGCGATG | |
| Mut iacEFw | AAGTCGTCTCCGTGATGGAC | Mut iacERv | GATCACCGCCTTCAGTTTGT | |
| Mut iacFFw | AACAGGAGCGCTTCGTATTG | Mut iacFRv | GCAAACCGTTCGAGATGAAT | |
| Mut iacGFw | TCGACGTCAAACAGCAATTC | Mut iacGRv | ACCGAAGCGTTCTTCCATC | |
| Mut iacHFw | ATCGGATTGAAGCCCACTTT | Mut iacHRv | CGAGGTCTCGACATTGATGA | |
| Mut iacIFw | GAAATTCTCGCGATGCTCAC | Mut iacIRv | GAAAGTGCGTGATGAACCAG | |
| Mut iacA2Fw | GTCGAGAACTGGAACGTGGT | Mut iacA2Rv | CAGTTGGCTTCTGCTTTCG | |
| Mut iacG2Fw | CCAGCTACGTGCATGACATC | Mut iacG2Rv | AATCTGCGCAAACATGACC | |
| Mut iacT1Fw | GGTGGAAAGCTTCATGTCGT | Mut iacT1Rv | GAGGAACACCAGCATTGGTT | |
| Mut iacT2Fw | AGCATTCTGCTGATTCACGA | Mut iacT2Rv | TTGAGAACGCGAAGAAGATG | |
| Mut iacYFw | ATTGATCGCGGCTCTCAC | Mut iacYRv | GCGATCAAGGCTTCTTTTTG | |
| Mut iacSFw | TCAGCGTGGAAGACACACTC | Mut iacSRv | GTTGTAACGTCCGGCGTATT | |
| Mut iacR1Fw | GTGCAAGCGGAGTTGAATC | Mut iacR1Rv | CTTCACGCTGATCGACAGAT | |
| Mut iacR2Fw | CGCTGGAACTGTCGACTATC | Mut iacR2Rv | GAAACGCCTTCAAACTCAGC | |
| Mut catAFw | TCTGGGTTTCGAGCATTTTC | Mut catARv | CGGAAGCTGTATTTGCCTTC | |
| RT-PCRa analysis | iacA intFw | GCCCAGTTTCTCGACATGAT | iacA intRv | AGATTTCCGCTTGTGTATGC |
| iacC intFw | GATGGTTATGAAGGCGTGGT | iacC intRv | GGCTCGATCTCCTGATTGAA | |
| iacF intFw | CTGATGCGATTCATCTCGAA | iacF intRv | ATGGTCGTGTCCTTGTCCAC | |
| iacG intFw | GCGACTTTCCAGAGCAACC | iacG intRv | ACCGAAGCGTTCTTCCATC | |
| iacA2 intFw | GTCGAGAACTGGAACGTGGT | iacA2 intRv | CAGTTGGCTTCTGCTTTCG | |
| iacR1intFw | GTGCAAGCGGAGTTGAATC | iacR1intRv | CTTCACGCTGATCGACAGAT | |
| iacR2intFw | CGCTGGAACTGTCGACTATC | iacR2intRv | GAAACGCCTTCAAACTCAGC | |
| iacSintFw | TTGTCCCTTCGTTTGTCCTT | iacSintRv | CCAGGTGGTGTTGAATCTGG | |
| catA intFw | ACGTACAACGGACCTTCGAT | catA intRv | TCTGGGTTTCGAGCATTTTC | |
| catA2 intFw | TTCAACAAGCTCGGACAGG | catA2 intRv | ACATACAGCGGACCTTCGAT | |
| kynA intFw | TGAAGCTCGCGTTGTATGAG | kynA intRv | TCCGATAAACCTCCAACCAG | |
| rpS7 intFw | CGAACAGATCCAGACCAAGG | rpS7 intRv | ACTTCTCGCTGCGCTTCTT | |
| 16S intFw | CGGGCTAGAGTATGGCAGAG | 16S intRv | CGTGCATGAGCGTCAGTATT | |
| Transcriptional fusions | PiacA Fw | GCGCGGTACCCAGGCGGTGAAAGTCTTCTT | PiacA Rv | TGTCCTCGAGGGTGAGGTATCTCCTTGATTCG |
| PiacC Fw | GCGCGGTACCGAATGAAAAGCGGGTCGTC | PiacC Rv | TCGCCTCGAGCTTGTGTAGGTGGGGGTGGT | |
| PiacF Fw | TAGAGGTACCGATTTGAATGTTCCGACTGGA | PiacF Rv | CGTTCTCGAGGGTTCTTTCTATGAGCTTGC | |
| PiacG Fw | TTATGGTACCCGAAGGCTGGGACATGGT | PiacG Rv | ATTTCTCGAGGATTCCTCGGGCAGGTGT | |
| PcatB Fw | GTTTGGTACCTTCCTCGATCTGCT GGATCT | PcatB Rv | ATTCCTCGAGCTCCTGAACTGCCTGACCTG | |
| iac gene expression | 1 (iacABIHE Fw) | TTGGGCTAGCGAATTCCTGCTTACGTTTCGATCCAACTTGC | 2 (iacABIHE Rv) | GTGGTCGATGAAGCGAAGTCGTCAGCGCATGAAGAGTCC |
| 3 (iacCD Fw) | GACTTCGCTTCATCGACCAC | 4 (iacCD Rv) | CAGGTGTCGAGTGGTTTGATTTCGAAAAGATCAAAGCAGGAA | |
| 5 (iacG Fw) | AATCAAACCACTCGACACCTG | 6 (iacG Rv) | GGTTGTGCGTCAAGGATAAGCCACTGTGCGTAATCAATGC | |
| 7 (iacF Fw) | CTTATCCTTGACGCACAACC | 8 (iacF Rv) | GTAATACGACTCACTATAGGAAGAAAAGGCGCTTGTGAAA | |
| 9 (pBS1 Fw) | CCTATAGTGAGTCGTATTAC | 10 (pBS1 Rv) | GCAGGAATTCGCTAGCCCAA | |
| 11 (iacB Fw) | TTGGGCTAGCGAATTCCTGCCTATCCGCACAACCAGGATT | 12 (iacA Rv) | AATCCTGGTTGTGCGGATAG | |
| 13 (iacI Fw) | CTATCCGCACAACCAGGATTCTTGAGCGTTTCGGAACAAC | 14 (iacH Rv) | GTGGTCGATGAAGCGAAGTCCCACTCCACTTCGAAATAACG | |
| 15 (iacE Rv) | CAGGTGTCGAGTGGTTTGATTATGCCGCTTTGGTGTTGT | 16 (iacG Rv2) | GTAATACGACTCACTATAGGCCACTGTGCGTAATCAATGC | |
| 17 (iacF Fw2) | TTCCTGCTTTGATCTTTTCGACTTATCCTTGACGCACAACC | 18 (iacI Rv) | CCTATCGACAGCGTCTGGA | |
| 19 (iacE Fw) | TCCAGACGCTGTCGATAGGGCCGTTATTTCGAAGTGGAG | 20 (iacB Rv) | TCCTGGTTCGCGTTCATT | |
| 21 (iacH Fw) | AATGAACGCGAACCAGGAAGGTCAACGCAAGGAAACC | 22 (iacA Rv2) | GTAATACGACTCACTATAGGAATCCTGGTTGTGCGGATA | |
| 23 (iacA Rv3) | CAGGTGTCGAGTGGTTTGATTAATCCTGGTTGTGCGGATAG | 24 (iacA2 Fw) | TTGGGCTAGCGAATTCCTGCAGCTTCTCCTGTACCGATCC | |
| 25 (iacA2 Rv) | GTAATACGACTCACTATAGGTTCCTTTTCCTTTTGCATGG | 26 (iacG2 Rv) | GTAATACGACTCACTATAGGGATGAAAGATCCGGAGAGCA | |
| 27 (iacE Fw2) | TTGGGCTAGCGAATTCCTGCGCCGTTATTTCGAAGTGGAG | 28 (iacE Rv2) | GTAATACGACTCACTATAGGATGCCGCTTTGGTGTTGT | |
| 29 (iacB Rv2) | GTAATACGACTCACTATAGGTCCTGGTTCGCGTTCATT | 30 (iacE Fw3) | AATGAACGCGAACCAGGAGCCGTTATTTCGAAGTGGAG | |
| 31 (iacC Fw) | TTGGGCTAGCGAATTCCTGCGACTTCGCTTCATCGACCAC | 32 (iacC Rv) | GTAATACGACTCACTATAGGAGCGTGTTTCTGTCGTCCAT | |
| 33 (iacD Rv) | GTAATACGACTCACTATAGGTCGAAAAGATCAAAGCAGGAA | 34 (iacF Rv1) | AAGAAAAGGCGCTTGTGAA | |
| 35 (iacI Fw2) | TTTCACAAGCGCCTTTTCTTCTTGAGCGTTTCGGAACAAC | 36 (iacI Rv2) | GTAATACGACTCACTATAGGCCTATCGACAGCGTCTGGA | |
| 37 (iacH Fw2) | TTGGGCTAGCGAATTCCTGCAGGTCAACGCAAGGAAACC | 38 (iacH Rv2) | GTAATACGACTCACTATAGGCCACTCCACTTCGAAATAACG | |
| 39 (iacY Fw) | ACAGGATGAGGATCGTTTCGATCGGCTTCCTGCTTTGAT | 40 (iacT1 Rv) | CAAGAAAGCTGGGTCGAATTGGCTCGAATGCAGCTTCTC | |
| 41 (pKan Fw) | CTAAGTAAGTAAGGAGAAGGGCGGGCTATCTGGACAAGGGAAA | 42 (pKan Rv) | CGAAACGATCCTCATCCTGT | |
| 43 (p0 Fw1) | AATTCGACCCAGCTTTCTTG | 44 (p0 Rv1) | CCTTTGAGTGAGCTGATACCG | |
| 45 (p0 Fw2) | CGGTATCAGCTCACTCAAAGG | 46 (p0 Rv2) | CGCCCTTCTCCTTACTTACTTAG | |
a
RT-PCR, reverse transcription-PCR.