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Detection of MPO in lesion HDL by 2D liquid chromatography tandem MS analysis. (A) Sequence of MPO. (B) HDL isolated from human lesions was digested with trypsin and subjected to liquid chromatography-electrospray ionization-MS/MS analysis. Four peptides (underlined in A) unique to MPO were identified. The MS/MS spectrum of one peptide (WDGERLYQEARK) from the heavy chain of MPO is shown.
