Fig. 5.

Cholesterol efflux activities of native and HOCl-oxidized HDL, apoA-I, and peptide 18A. [³H]Cholesterol-labeled mock- (A and C) or ABCA1-transfected BHK cells were incubated for 4 h with native (Ctrl), H₂O₂-oxidized, HOCl-oxidized HDL (20 μg/ml), or apoA-I (5 μg/ml) (A), for 2 h with 5 μg/ml apoA-I oxidized with the indicated mol ratio of HOCl (B) or for 2 h with control (-) or HOCl-oxidized (+) peptide Ac-18A-NH₂ (20 μg/ml) (C). At the end of the incubation, [³H]cholesterol efflux to the acceptor particle was measured. ^*, P < 0.01 compared with controls.