Table 2.
Repeatability and reproducibility of the HIV-1 RNA-based Amplix real-time PCR assay.
| Panel | Target HIV-1 RNA (log copies/mL) | Number of determinations | Mean (SD) (log copies/mL) of HIV-1 RNA measured | Coefficient of variation (%) |
|---|---|---|---|---|
| Repeatability | ||||
| Third WHO standard | 5.04 | 3 | 5.2 (0.3) | 5.7 |
| Low-positive pool | 3.17 | 30 | 3.2 (0.2) | 7.7 |
| High-positive pool | 6.33 | 30 | 6.5 (0.3) | 4.6 |
|
| ||||
| Reproducibility | ||||
| Third WHO standard | 5.04 | 3 | 5.3 (0.4) | 7.5 |
| Low-positive pool | 3.17 | 30 | 3.3 (0.3) | 9.1 |
| High-positive pool | 6.33 | 30 | 6.6 (0.4) | 6.0 |
SD: standard deviation.