FIG 12.

CO reduces the inflammatory cytokine mRNA levels in PRRSV-infected MARC-145 cells and PAMs. (A to D) MARC-145 cells were infected with PRRSV (MOI of 0.1) and incubated in the presence or absence of various doses of CORM-2 or iCORM-2. Total RNA was extracted from cell lysates at 24 hpi. The relative expression levels of IL-6 mRNA (A), IL-8 mRNA (B), TNF-α mRNA (C), and IL-1β mRNA (D) were assessed by qPCR. Values were normalized to the internal control β-actin. (E to H) PAMs were treated as described above. The relative expression levels of IL-6 mRNA (E), IL-8 mRNA (F), TNF-α mRNA (G), and IL-1β mRNA (H) were each assessed by qPCR. Values were normalized to the internal control β-actin. The data are representative of three independent experiments performed in triplicate and are shown as the means ± SD. Statistical analysis was performed using ANOVA.*, P < 0.05; **, P < 0.01; ***, P < 0.001.