FIG 9.

CO inhibits NF-κB-responsive promoter activity. MARC-145 and CRL2843 cells were transfected with 100 ng/well of pNF-κB-Luc and 50 ng/well of pRL-TK. (A) At 12 h posttransfection, MARC-145 cells were infected with PRRSV at an MOI of 0.1, followed by treatment with various concentrations of CORM-2 or 150 μM iCORM-2 from 1 hpi onward; 36 h after PRRSV infection, cells were lysed for luciferase activity detection. (B) CRL2843 cells were treated with LPS in the presence of various doses of CORM-2 or 150 μM iCORM-2 12 h after transfection. After treatment with LPS for 36 h, the cells were lysed and luciferase activity was measured. Data are expressed as the means ± standard deviations of the results of four independent experiments; P values were calculated using ANOVA. *, P < 0.05; **, P < 0.01; ***, P < 0.001.