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. 2016 Dec 16;91(1):e01866-16. doi: 10.1128/JVI.01866-16

TABLE 1.

Sequences of the primers used for qPCR

Primer Sequence (5′–3′)
PRRSV ORF7 gene Forward, AGATCATCGCCCAACAAAAC
Reverse, GACACAATTGCCGCTCACTA
PRRSV NSP2 gene Forward, GTGGGTCGGCACCAGTT
Reverse, GACGCAGACAAATCCAGAGG
β-Actin Forward, TCCCTGGAGAAGAGCTACGA
Reverse, AGCACTGTGTTGGCGTACAG
mIL-6 Forward, AGAGGCACTGGCAGAAAAC
Reverse, TGCAGGAACTGGATCAGGAC
mIL-8 Forward, AGGACAAGAGCCAGGAAGAA
Reverse, ACTGCACCTTCACACAGAGC
mTNF-α Forward, TCTGTCTGCTGCACTTTGGAGTGA
Reverse, TTGAGGGTTTGCTACAACATGGGC
mIL-1β Forward, GGAAGACAAATTGCATGG
Reverse, CCCAACTGGTACATCAGCAC
pIL-6 Forward, AATGTCGAGGCTGTGCAGATT
Reverse, TGGTGGCTTTGTCTGGATTCT
pIL-8 Forward, CACTGTGAAAATTCAGAAATCATTGTTA
Reverse, CTTCACAAATACCTGCACAACCTTC
pTNF-α Forward, TGGTGGTGCCGACAGATGG
Reverse, GGCTGATGGTGTGAGTGAGGAA
pIL-1β Forward, ACCTGGACCTTGGTTCTCTG
Reverse, CATCTGCCTGATGCTCTTGT
hVP1 Forward, CTGGTAAAGGTCCAGCACTC
Reverse, GGGAGGTCTATCTCTCCAAC