FIG 5.

Depletion of SETDB1 but not TRIM28 relieves MLV transcriptional repression in human EC cells. (A) Flow cytometry analysis of indicated 2102Ep or F9 cells infected with VSV-G-pseudotyped MLV-GFP reporter viruses; this is at 2 days postinfection. The y axis shows side scatter (SSC); the x axis shows GFP intensity. Results of one representative experiment of three independent experiments are shown. (B) Quantification of the results from panel A from multiple experiments. Results shown are means ± SEM from three independent experiments. (C) Lysates prepared from 2102Ep or F9 cells stably transduced with either scrambled shRNA or TRIM28- or SETDB1-specific shRNAs were subjected to Western blotting as indicated. (D) 2102Ep cells infected with MLV-GFP for 3 days were subjected to ChIP analysis using antibodies against H3K9me3. ChIP data are presented as the percentage of input DNA. Results shown are means ± SDs from two independent experiments performed in duplicate. (E) Experiments similar to those in panel D but using antibodies against acetylated histone H3 (H3Ac).