FIG 3.

Effects of nucleotide substitutions at 3′-end positions 13 to 15 and 5′-end positions 14′ to 16′ on protein productions in the RNP reconstitution system. (A, C, E, and G) Western blotting to examine the HA proteins expressed by the wild-type and mutant RNPs (the same as above). The wild-type and mutant RNPs were reconstituted in 293T cells individually, cells were harvested at 24 h posttransfection, and lysates were analyzed by Western blotting; β-actin was detected as a loading control. (B, D, F, and H) Quantification of the HA protein levels obtained from the experiments shown in panels A, C, E, and G by densitometry analysis, respectively. The values were expressed as percentages relative to the level of the HA protein expressed from the wild-type RNP system. Data in panels B, D, F, and H represent the means ± standard deviations of three independent experiments. *, P < 0.05; ***, P < 0.001; ns, nonsignificant.