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Optimization of storage conditions for the BTV1 DISC virus. (A) Virus titers in the presence of peptone, lactose, or trehalose at the indicated temperature (4°C, −20°C, or 24°C) at 4 and 28 days. Titers were expressed as PFU per milliliter and plotted in logarithmic scale. (B) Virus titers after desiccation in the presence of peptone, lactose, or two concentrations of trehalose (5% and 10%). No additive (PBS) and 4°C storage were included for comparison.
