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Long protection test in sheep vaccinated with a MultiDISC vaccine. (A) Detection of group-specific antibody response (against BTV VP7) using competition ELISA. Animals were vaccinated twice 21 days apart (empty arrows) and challenged 154 dpv (full arrow). (B) Virus load was determined by RT-PCR, and the results were expressed as mean CT values. The dashed line represents the PCR cutoff; results below the line are considered negative.
