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. 2016 Dec 16;91(1):e01613-16. doi: 10.1128/JVI.01613-16

FIG 1.

FIG 1

FIG 1

Regulation of syncytium formation by the gBcyt in VZV-infected cells. Live-cell confocal microscopy images of LifeAct-tGFP melanoma cells infected with pOka-TK-RFP (A) or gB[Y881F]-TK-RFP (B) captured from Movies S1 and S2 at https://purl.stanford.edu/wc992yg2549 at five time points, beginning at 24 hpi (T0) and at 5 (T5), 10 (T10), 15 (T15) and 20 (T20) hpi, are shown. Infected cells are detected by the RFP signal (red), actin filaments express LifeAct-tGFP (green), and nuclei are stained with Hoechst 33342 (blue). White boxes in the panels in the first column (1 to 3) indicate three areas within the monolayer that are shown at higher magnification in the columns at the right, labeled 1 to 3, for each of the five time points, (T0, T5, T10, T15, and T20). Arrowheads identify representative cells that have not undergone fusion within the syncytia (open arrowheads) or nuclei not expressing RFP (closed arrowheads). These images highlight events in cell-cell fusion that are visualized in Movies S1 and S2, available at https://purl.stanford.edu/wc992yg2549.