FIG 5.
Naf1 knockdown promotes viral reactivation from latently HIV-1-infected TCM. Latently HIV-eGFP/VSV-G-infected TCM were infected with lentiviruses containing Naf1-specific shRNA or a scramble control for 72 h (A) and then were stimulated with anti-CD3/CD28 antibodies for an additional 72 h, viral reactivation was measured by detecting GFP expression (B), and the percentage of GFP+ cells and the MFI were calculated (C). For panels B and C, data from 4 independent repeats were summarized and analyzed. **, P < 0.01 (paired t test). For panel A, the densities of bands were analyzed with the plug-ins of ImageJ software, and the values relative to that for GAPDH were calculated.