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HBoV1 does not infect HEK293 cells. HEK293 cells were mock infected (A) or infected with HBoV1 at an MOI of ∼400 vgc/cell (B). At 3 days postinfection, infected cells were analyzed by IF staining with anti-NS1C and anti-Ki67 antibodies. Nuclei were stained with DAPI, and confocal images were captured at a ×60 magnification.
