FIG 4.

Retro94 alters morphology of the HCMV assembly compartment. (A) Western blot analysis of uninfected cells (Mock) or HCMV-infected cells (AD169; MOI of 3) treated with DMSO or Retro94 (10 μM) at 24, 48, 72, and 96 hpi. (B) DNA replication analysis of cells infected at an MOI of 3 and harvested at 24, 48, 72, and 96 hpi. Samples were treated with DMSO, Retro94 (10 μM), or acyclovir (100 μg/ml). Data are composites of three independent replicates. (C) Localization of viral proteins in HCMV-infected cells at 96 hpi in the presence of DMSO or Retro 94 (10 μM). pp28 was detected by tagging with mCherry, and gB was detected by immunofluorescence. (D) Immunofluorescence detection of STX5 (green) in HCMV-infected cells (MOI of 3) at 96 hpi treated with DMSO or Retro94. pp28-mCherry is shown in red for reference. (E) Virus titers at 96 hpi of HCMV-infected cells treated with Retro94 at 2, 24, 48, or 72 hpi. *, P < 0.05; N.S., not significant. (F) Localization of pp28-mCherry in samples corresponding to those shown in panel E. (G) Immunofluorescence detection of pp28 in HCMV-infected cells subjected to either treatment with Retro94 throughout infection or treatment with Retro94 and subsequent withdrawal of Retro94 at 9 dpi followed by recovery for an additional 9 days. Nuclei shown in panels C, D, F, and G are labeled with DAPI (blue). Scale bar, 10 μm.