FIG 6.

Juxtanuclear accumulation of Golgi fragments in the absence of STX5. (A) Immunofluorescence of Golgi complex (GM130) and TGN (p230) markers (green) in HCMV-infected cells (AD169; MOI of 3, 96 hpi) treated with DMSO or Retro94. Lower insets show single cells enlarged from the images above. A merged image with pp28-mCherry (red) is shown for reference. (B) Immunofluorescence of Golgi apparatus markers p115 (green) and pp28 (red) in cells infected with miSTX5 in the presence or absence of doxycycline at 96 hpi. (C) Proposed model for the role of STX5 during HCMV infection. Nuclei shown in panels A and B are labeled with DAPI (blue). Scale bar, 10 μm.