FIG 7.

The Retro94-dependent inhibition of HCMV is not strain, cell type, or species specific. (A) Viral titers at 15 dpi of fibroblasts infected with various strains of HCMV and treated with DMSO or Retro94 at 2 hpi. The medium was changed, and fresh Retro94 was added at 6 dpi. Bright-field images representing CPE of infections are shown on the right. (B) HCMV-infected ARPE-19 cells treated with DMSO or Retro94 (10 μM). Infected cells were detected by monitoring mCherry (red) expressed by TB40 virus. Scale bar, 1 mm. The table below the images shows the number of foci detected, average area, and standard error of the mean (SEM). (C) Immunofluorescence analysis of STX5 (green) and pp28 (red) in HCMV-infected ARPE-19 cells at 96 hpi in the presence of DMSO or Retro94 (10 μM). (D) Viral titers at 6 dpi of MCMV-infected fibroblasts treated with DMSO or Retro94. (E) Immunofluorescence analysis of STX5 (green) and GM130 (red) in MCMV-infected NIH 3T3 cells at 72 hpi in the presence of DMSO or Retro94 (10 μM). (F) Growth curve analysis of HCMV (MOI of 3) in the DMSO control (No drug) or in cells treated with Retro94 and F-Retro94 at increasing concentrations. Samples were harvested at 96 hpi. Representative data shown are from one of four (Retro94) or one of three (F-Retro94) independent replicates. Values in A and D are composites of three independent experiments.