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. 2016 Dec 16;91(1):e01445-16. doi: 10.1128/JVI.01445-16

FIG 8.

FIG 8

Application of the artificial tail anchor strategy to optimize a peptidic MERS-CoV fusion inhibitor. (A) Schematic representation of the functional domains in the S2 subunit of MERS-CoV spike protein and sequences of M0 and M1 peptides. (B) Structural prediction for binding of M0 (a) or M1 (b) peptide with HR1 domain of MERS-CoV S protein. HR1 is colored in gray, the M0 peptide in green, and the EAN hook in magenta. The original sequence of MERS-CoV HR1 aa 1254 to 1256 is colored in cyan for comparison. The EAN hook is superposed on M0 in panel c and shown for residue interactions in panel d. (C) Inhibitory activities of M0 and M1 peptides against MERS-CoV S-mediated cell-cell fusion. (D) Inhibitory activities of M0 and M1 peptides against MERS pseudovirus infection. Error bars show standard deviations.