TABLE 2.

Constructed MERS-CoV RBD fragments containing single or multiple mutations in the RBD of representative MERS-CoV strains isolated in 2012 to 2015 and multiple key mutations in the RBM of the RBD (residues 377 to 588)^a

Protein	Yr isolated	Host	Mutation in MERS-CoV RBD residue:																	No. of mutations
			400	424	431	434	457	460	482	506	509	510	511	513	520	522	530	534	582
EMC-RBD	2012	Human	K	T	A	A	S	S	A	L	D	D	R	E	A	Q	V	V	N
2012-RBD	2012	Human								F	G							A		3
2013-RBD	2013	Human			P	V		F	V										I	5
2014-RBD	2014	Human		I												H				2
2015-RBD	2015	Human															L			1
Camel-RBD	2013	Camel	N			S	G								S					4
RBD-FGG	2012	Human								F	G									3
	2015	Human										G
RBD-FGGAA	2012	Human								F	G									5
	2015	Human										G
		Escape mutants											A
														A

^aEMC-RBD, RBD fragment constructed based on the sequence of MERS-CoV EMC2012 (prototype) strain. Key residues in the RBM of the RBD are in bold.