TABLE 1.
Data collection and refinement statistics of arterivirus nsp11 mutantsa
| Parameter | Value for: |
||
|---|---|---|---|
| PRRSV K173A mutant (PDB code 5EYI) | EAV K170A mutant (PDB code 5HBZ) | EAV H141A mutant (PDB code 5HC1) | |
| Data collection statistics | |||
| Wavelength | 0.9793 | 1.0000 | 1.0000 |
| Space group | P41212 | R32 | I222 |
| Cell dimensions | |||
| a, b, c (Å) | 75.3, 75.3, 199.5 | 248.3, 248.3, 226.3 | 129.1, 133.7, 145.3 |
| α, β, γ (°) | 90, 90, 90 | 90, 90, 120 | 90, 90, 90 |
| Resolution (Å) | 50.0–2.16 (2.20–2.16) | 50.0–3.10 (3.15–3.10) | 50.0–3.10 (3.15–3.10) |
| Rsym (%)b | 9.9 | 10 | 26.6 |
| I/σ | 18.4 (1.5) | 15.7 (1.5) | 6.2 (1.6) |
| Completeness (%) | 99.6 (99.9) | 99.2 (99.5) | 90.7 (93.3) |
| Total no. of reflections | 1,657,775 | 1,174,148 | 793,92 |
| Unique reflections | 31,952 | 48,540 | 23,202 |
| Redundancy | 7.7 (7.9) | 5.5 (5.7) | 7.7 (7.7) |
| Refinement statistics | |||
| Resolution (Å) | 50.0–2.16 (2.21–2.16) | 50.0–3.10 (3.18–3.10) | 50.0–3.10 (3.18–3.10) |
| No. of reflections | 297,12 (1863) | 44,766 (2699) | 19,651 (1397) |
| Rwork/Rfree (%) | 18.4/21.0 | 19.5/19.9 | 20.8/25.2 |
| No. of atoms | |||
| Protein | 3,369 | 9,946 | 6,658 |
| Ligand/ions | 14 | 0 | 0 |
| Water | 440 | 0 | 125 |
| B-factors (Å2) | |||
| Protein | 29.1 | 73.9 | 66.3 |
| Ligand/ion | 50.4 | 0 | 0 |
| Water | 42.2 | 0 | 39.4 |
| RMSDs | |||
| Bond lengths (Å) | 0.007 | 0.009 | 0.007 |
| Bond angles (°) | 1.34 | 1.438 | 1.28 |
| Ramachandran plot (%)c | 91.5/8.5/0/0 | 84.8/14.7/0.5/0 | 83.8/15.3/0.9/0 |
a
Three crystal experiments for each structure.
b
Rsym = ΣhΣi|Ih,I − Ih|/ΣhΣlIh,i, where Ih is the mean intensity of the i observations of symmetry-related reflections of h.
c
Residues in most favored, additional allowed, generously allowed, and disallowed regions of the Ramachandran plot.