FIGURE 2.

Analyses of anthocyanidin synthase alleles in cv. “Tulameen” and “Anne”. Comparison indicate a 5 bp insertion (ans⁺⁵) in the second exon at position 730 of “Anne” allele (ans) causing a frameshift and thus leading to a truncated protein in the yellow variety, missing conserved regions for substrate-, co-substrate, and iron binding sites. The Ans fragment from “Anne” and “Tulameen” includes the entire coding region with one 446 bp intron at position 509, and 118 bp of the 5′UTR and 29 bp of the 3′UTR.