Table 1. d-ghrelin-binding sequences identified by in vitro selection and sequencing.

Clone	Selected sequences, variable region	Frequency	Kd, nM
B11	UG---AGGCAAU------------AAAACU--UAAGUCCGAAGGUAACCAAUCCUA	65×	44.4
G2	UG---AGGCAGU------------AAAACU--UAAGUCCGAAGGUAACCAAUCCUA	1×
E12	UG---AGGCAAU------------AAAACU--UAAGUCCGAAGGUAACCAAUCCUG	1×
B7	UG---AGGCAAU------------AAAACA--UAAGUCCGAAGGUAACCAAUCCUA	2×
A8	UG---AGGCAAU------------AAAACG--UAAGUCCGAAGGUAACCAAUCCUA	2×
B12	UG---AGGCAAU------------AAAACUUGUAAGUCCGAAGGUAACCAAUCCUA	1×
E3	UG---AGGCAAUA-----------AAAACU--UAAGUCCGAAGGUAACCAAUCCUA	5×	89.9
C11	UG---AGGUAGUAAA---------AAAACG--UAAAUCCGAAGGUAACCAAUCCUA	2×
A3	UG---AGGUAGUAAAAAA------AAAACG--UAAAUCCGAAGGUAACCAAUCCUA	2×
F5	UG---AGGUAGUAAAAAAA-----AAAACG--UAAAUCCGAAGGUAACCAGUCCUA	1×
A12	UG---AGGUAGUAAAAAAAA----AAAACG--UAAAUCCGAAGGUAACCAAUCCUA	1×
F12	UG---AGGUAGUAAAAAAAAA---AAAACG--UAAAUCCGAAGGUAACCAAUCCUA	2×	87
G5	UG---AGGUAGUAAAAAAAAAAAAAAAACA--UAAAUCCGAAGGUAACCAAUCCUA	1×
C12	UGGUGAGGCA--------------AAAACG--UAAGACCGAAGGUAACCAUUCCUA	1×

After 17 selection rounds, 87 full-length clones were sequenced. All sequences belonged to a single family, with sequence B11 being the most common representative. Other sequences differed by point mutations or insertions (in bold). Binding to biotinylated d-ghrelin was determined for the three most frequent sequences by using pull-down assays.