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. 2004 Aug 30;101(36):13233–13238. doi: 10.1073/pnas.0405507101

Fig. 3.

Fig. 3.

paf-2(tj12) mutants display epidermal abnormalities. (A and B) Scanning electron micrographs of wild-type (A) and paf-2(tj12)(B) embryos at the terminal stage. In the wild-type embryo, dorsal (#), lateral (+), and ventral (asterisk) rows of epidermal cells are visible (A, lateral view), whereas paf-2(tj12) embryos show aberrant surface structure (B, presumably the dorsal view). (C–E) Comparison of three-dimensional reconstructions of MH27 immunostaining in wild-type (C, lateral view) and terminal stage paf-2(tj12) embryos (D, lateral view; E, dorsal view). In terminal paf-2(tj12) embryos, the MH27 staining is punctate and irregular or is absent in many parts of the epidermis, especially on the dorsal side (D, asterisk; see text). Note that the MH27 staining of intestinal cells is also discontinuous in the paf-2(tj12) embryos (E, yellow line), whereas the staining is continuous in the wild-type (C, arrow). (F–I) Embryos were costained with MH27 (red) and LIN-26 antiserum (green). (F and G) Ventral enclosure. In the wild-type embryo, the epidermis fully enclosed the embryo (F, ventrolateral external focal plane showing the ventral epidermal cells). In paf-2(tj12) embryos, the shapes and patterning of epidermal cells are aberrant and the incomplete epidermal sheet fails to fully enclose the embryo (G, lateral external focal plane). (H and I) Dorsal intercalation (dorsal external focal planes showing the dorsal epidermal cells). In wild-type embryos, two rows of dorsal epidermal cells have intercalated to form a single row (H, arrow), whereas the dorsal epidermal cells in the paf-2(tj12) embryo were mislocalized and MH27 staining soon diminished at the cell junction (I, asterisk). Arrowheads in H indicate the lateral epidermal cells. (J and K) Embryos were stained with MH33, which specifically recognizes intestinal cells (internal focal planes, lateral view). Comparison of wild-type embryos (J, twofold stage) and paf-2(tj12) embryos (K, terminal lima bean stage). The intestinal cells of the paf-2(tj12) embryo appear to be differentiated at least partially (K). Anterior is to the left in all images. (Bar, 10 μm.)