Fig. 1.

In vitro induction of PLSCR3^-/- granulocytes and macrophages from hematopoietic precursors. Shown are Wright-Giemsa-stained cultures of bone marrow cells after culture to induce differentiation and maturation to either granulocytes (a) or macrophages (b) as detailed in Methods (×400). Fields shown are representative of >100 surveyed from each of at least three independent experiments. (a) Note prominence of immature-appearing nuclei in association with the PLSCR1^-/- genotype and the increase in frequency of cells displaying cytoplasmic lipidoid inclusions in association with the PLSCR3^-/- genotype. Of 2,000 cells counted in multiple random fields, the % of cells exhibiting such lipoid inclusions was: WT, 0.8%; PLSCR1^-/-, 0.9%; PLSCR3^-/-, 2.2%; PLSCR(1&3)^-/-, 3.4%. (b) Prevalence of cells with apparent lipoid cytoplasmic inclusions in association with the PLSCR3^-/- genotype (compare Table 2).