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Detection of hypoxia in the EndoC-βH3 aggregated cells when cultured under 3% O2. To detect hypoxic cells, pimonidazole (200 μmol/L; Hypoxyprobe; NPI) was added to the culture medium during the last hour of the culture period (6 days). PIs were analyzed with an anti-pimonidazole antibody (NPI). Control cells were cultured under normoxia.
