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. Author manuscript; available in PMC: 2016 Dec 19.
Published in final edited form as: Protein J. 2016 Jun;35(3):179–192. doi: 10.1007/s10930-016-9659-9

Figure 5.

Figure 5

Vimentin Phosphorylations. 2D SDS-PAGE and Western blot analysis, followed by mass spectrometry shows that vimentin undergoes site-specific phosphorylations and depolymerization under stress conditions. Immunocytochemistry demonstrates stress-induced vimentin aggregation and depolymerization (short filaments). A scaffold subunit of PP2A (#1 box) and vimentin (#2 box) were upregulated under constant light, 1.8 and 2 fold, respectively (7 days) (Figure 5A). Target proteins were detected using Coomassie blue staining, Western blotting (Figure 5B), and analyzed by MALDI-TOF-TOF mass spectrometry (Table 2).