FIG. 6.

IFN-λ induces expression of the antiviral genes CXCL10, ISG15, and PKR in peripheral blood immune populations. BDCA-4⁺ pDCs, CD19⁺ B cells, CD14⁺ monocytes, and CD3⁺ T cells were isolated from peripheral blood mononuclear cells (PBMCs) by magnetic bead separation and cultured either untreated or treated with (A) IFN-λ1 (100 ng/mL) or (B) IFN-α (1,000 U/mL) for 4 h. Following RNA extraction, CXCL10, ISG15, and PKR expression was measured using real-time PCR. Fold change is shown relative to the untreated sample for each cell type (as indicated by dotted line) and values were normalized to the mean of 2 housekeeping genes GAPDH and RPS15 using the ΔΔCt method. Each bar represents the mean of n = 5–6 blood donors for B cells, T cells, and monocytes and n = 3 donors for pDCs. Error bars show SEM.