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. 2016 Dec 1;35(12):828–835. doi: 10.1089/dna.2015.3186

FIG. 4.

FIG. 4.

p65 is a target gene of miR-372 in DU145 cells. (A) Western blot analysis was used to detect the expression of p65, CDK8, PSA, and MMP9 in DU145 cells after treatment with miR-497, siR-p65, and control. The expression of p65 and IKKβ was also detected after transfection with siR-p65 and siR-IKKβ to clear the relationship between P65 and IKKβ in PCa. β-actin served as an internal control. (B) Relative luciferase activity of the p65 WT-UTR and the DEL-UTR luciferase constructs in DU145 cells transfected with miR-372 or control (*p < 0.05). (C) p65 was predicted to be a candidate target of miR-372 using the bioinformatics algorithm TargetScan. PSA, prostate-specific antigen.