FIG. 2.

Study design. Control embryos were cultured undisturbed up to fully expanded blastocyst stage, and SBM was collected before thawing. All seven blastocysts were diagnosed as euploid and healthy pregnancies ensued after transfer. Twin embryos instead were produced after embryo splitting at the cleavage stage. Twin A were cultured up to the blastocyst stage in their own zona pellucida and twin B were cultured in host zona pellucida. SBM was collected at the blastocyst stage. miRNAs were extracted and analyzed from seven controls and seven pairs of twins. miRNAs, microRNAs; SBM, spent blastocyst medium.