Fig. 6.

Dasm1 lacking the intracellular tail acts as a dominant negative and impairs dendrite outgrowth. (A and B) Neurons expressing Dasm1(delC)-EGFP (B) showed defects in dendrite development when compared with control neurons expressing EGFP alone (A). The dendritic arbor was visualized with MAP2 antibody staining (Center). The EGFP channel is shown in Left, and the overlay is shown in Right. (Scale bar = 50 μm.) (C) The average total dendritic branch length was decreased in neurons expressing Dasm1(delC)-EGFP or enhanced cyan fluorescent protein (ECFP)/Dasm1(delC)-EGFP compared with control neurons expressing EGFP or ECFP alone (^**, P < 0.001). (D) Sholl analysis showed that the dendritic arbor was severely reduced in neurons expressing Dasm1(delC)-EGFP (n = 22) compared with control neurons expressing EGFP alone (n = 21). Concentric circles with a 20-μm spacing were drawn around the cell body, and the number of intersections of all dendritic branches with the circles was counted.