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. 2004 Aug 23;101(36):13368–13373. doi: 10.1073/pnas.0403453101

Fig. 6.

Fig. 6.

Immunoblots of proteins in human urine exosomes (Urine) and kidney regions (Cortex, Outer Medulla, and Inner Medulla). Immunoblots were probed with antibodies to the following proteins: ACE, angiotensin-converting enzyme; ALIX, ALG-2 interacting protein X; APN; AQP1; AQP2; CA4, carbonic anhydrase type IV; CD9; CLIC1, chloride intracellular channel-1; αENaC, epithelial sodium channel α; βENaC, epithelial sodium channel β; γENaC, epithelial sodium channel γ; MUC1, mucin-1; NCC; NKCC2, Na-K-2Cl cotransporter type 2; PODXL, podocalyxin; RAB4; RAB5B; RAB11; SNX18, sorting nexin-18; sorcin; TSG101; and PC1, polycystin-1. In the PC1 immunoblot, an additional lane was added (PC1+), which was loaded with PC1 protein extracted from Madin-Darby canine kidney cells with stable expression of human PC1 protein (a kind gift of Feng Qian, The Johns Hopkins University, Baltimore).