The phenotypes of Atgif1 mutant and AtGIF1-RNAi transgenic plants. (A) Representation of the gif1 T-DNA insertional mutation. Black boxes, exons; solid line, introns; inverse triangle, T-DNA integration site. The ruler is a scale of base pairs. (B) RT-PCR determination of GIF1 mRNA content in the mutant. Amplification of actin mRNA was used as control. (C) Col and gif1 plants (Left) and rosette leaves from the first to fourth leaf 20 d after germination (Right). (D) Dimensional parameters of the first two rosette leaves from Col and gif1 plants at 20 d. (E and F) Col and gif1 flowers and petals (E) and their dimensional parameters (F). (G and H) Dimensional parameters of the first two rosette leaves (G) and dimensional parameters of petals (H) from Ws and GIF1-RNAi plants. C, Col; g, gif1; W, Ws; R, GIF1-RNAi line 7; T, the grf1-2 grf2 grf3 triple mutant; and 5, 7, 8, GIF1-RNAi lines. Error bars indicate standard error.