FIG. 2.

rMan5K produced by E. coli. (A) Purified proteins analyzed by SDS-PAGE and stained with Coomassie blue. Lane 1, fraction of Man5K eluted with imidazole from Ni-nitrilotriacetic acid agarose; lanes 2 and 3, rFL-Man5K and rT-Man5K separated by gel filtration chromatography, respectively. (B) Specific activities of rFL-Man5K (FL) and rT-Man5K (T) with galactomannan substrates (0.5% LBG and 0.5% guar gum). (C) N-terminal sequence of rFL-Man5K. The arrows indicated the two major cleavage sites leading to rT-Man5K. DS 1 and DS 2, duplicated sequences 1 and 2.