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. 2004 Oct;186(19):6586–6594. doi: 10.1128/JB.186.19.6586-6594.2004

TABLE 3.

Symbiotic characteristics of reporter GLF derivative strainsa

Strain Fix pheno- type Plants
Bacteroids
Color Dry wt (g/pot)b Nitro- genase activityc β-Galacto- sidase activity (Miller units)
GLF1 Fix+ Green (wild type) 4.85 ± 0.3 5.1 ± 1.6 1,021 ± 30
GLF2 Fix+ Intermediate 2.65 ± 0.25 1.9 ± 0.6 267 ± 65
GLF3 Fix+ Intermediate 2.25 ± 0.25 1.8 ± 0.45 172 ± 80
GLF4 Fix Yellow 1.15 ± 0.25 <10 NDd
GLF8 Fix Yellow 1.20 ± 0.32 <10 ND
GLF12 Fix+ Intermediate 2.67 ± 0.35 1.9 ± 0.7 40 ± 20
GLF18 Fix Yellow 1.12 ± 0.2 <10 ND
a

The strains were used as inoculants for peas, and the Fix phenotypes, plant colors, dry weights 32 days after inoculation, and nitrogenase and β-galactosidase activities of pea bacteroids were determined. All values are averages ± standard deviations for three assays.

b

Each pot contained five plants.

c

Nitrogenase activity is expressed in nanomoles of C2H2 reduced per hour per plant.

d

ND, not determined.