TABLE 3.
Strain | Fix pheno- type | Plants
|
Bacteroids
|
||
---|---|---|---|---|---|
Color | Dry wt (g/pot)b | Nitro- genase activityc | β-Galacto- sidase activity (Miller units) | ||
GLF1 | Fix+ | Green (wild type) | 4.85 ± 0.3 | 5.1 ± 1.6 | 1,021 ± 30 |
GLF2 | Fix+ | Intermediate | 2.65 ± 0.25 | 1.9 ± 0.6 | 267 ± 65 |
GLF3 | Fix+ | Intermediate | 2.25 ± 0.25 | 1.8 ± 0.45 | 172 ± 80 |
GLF4 | Fix− | Yellow | 1.15 ± 0.25 | <10 | NDd |
GLF8 | Fix− | Yellow | 1.20 ± 0.32 | <10 | ND |
GLF12 | Fix+ | Intermediate | 2.67 ± 0.35 | 1.9 ± 0.7 | 40 ± 20 |
GLF18 | Fix− | Yellow | 1.12 ± 0.2 | <10 | ND |
The strains were used as inoculants for peas, and the Fix phenotypes, plant colors, dry weights 32 days after inoculation, and nitrogenase and β-galactosidase activities of pea bacteroids were determined. All values are averages ± standard deviations for three assays.
Each pot contained five plants.
Nitrogenase activity is expressed in nanomoles of C2H2 reduced per hour per plant.
ND, not determined.