FIG. 1.

PRPP- and ribose 1,5-bisphosphate-dependent ¹⁴CO₂ fixation into an acid-stable product by extracts of M. jannaschii. (A) PRPP- and ribose 1,5-bisphosphate (R1,5-P)-dependent ¹⁴CO₂ fixation in the presence (grey bars) and in the absence (open bars) of the RubisCO transition state inhibitor CABP. (B) RubisCO dependence of acid-stable ¹⁴CO₂ incorporation in extracts of M. jannaschii. The open bars show the results obtained under normal assay conditions. The gray bars show the results of assays performed in the presence of antibody generated against recombinant M. jannaschii RubisCO with mixtures incubated as described in Materials and Methods. All assays were performed for 60 min.