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Plate assay of the activation of Mpr and MprH6(S93E) in different multiple extracellular protease gene-deficient B. subtilis strains. The activation of pro-Mpr was monitored by clear zones around B. subtilis colonies oversecreting Mpr for the indicated time. Lane 1, B. subtilis strain carrying pSP704 (negative control); lane 2, pMpr; lane 3, pMprH6; lane 4, pMprH6(S93E).
