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In vitro processing of pro-Mpr by Bpr. SDS-PAGE analysis (A) and artificial substrate colorimetric assay (B) of purified pro-Mpr processed by purified Bpr in vitro. Purified pro-Mpr (3.3 μg) was added to each mixture (100 μl) in the results shown in lanes 2 to 5. Lane 1, 4.5 μg of purified Bpr; lane 2, 4.5 μg of Bpr; lane 3, 1.5 μg of Bpr; lane 4, 0.75 μg of Bpr; lane 5, pro-Mpr. b, Bpr; p, pro-Mpr; m, mature Mpr; Abs410nm, absorbance at 410 nm..
