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Characterization of processing and activation of Mpr(S93E) in different B. subtilis strains. SDS-PAGE analysis (A), Western blotting (B), and artificial substrate colorimetric assay (C) of the culture supernatants of different B. subtilis strains carrying pMprH6(S93E). Lane 1, B. subtilis 168; lane 2, DB104; lane 3, SB300; lane 4, PB300; lane 5, DB428; lane 6, LB700. p, precursor; m, mature.
