FIG. 3.

Stationary-phase-induced reversions for his (A), met (B), and leu (C) alleles of MPRYB150 (pDG148) (•), MPRYB148 (pDG148-mutL) (♦), MPRYB149 (pDG148-mutS) (▪), and MPRYB156 (pDG148-mutSL) (▴) B. subtilis strains. As described in Materials and Methods, bacteria were grown in NOB-YE medium containing KAN (10 μg/ml) to an optical density at 600 nm of 0.5, and then IPTG to a final concentration of 5 mM was added to the cultures. At 150 min past T₀ the strains were plated in minimum medium containing 2.5 mM IPTG. Results are the averages from six different selection plates. Each experiment was performed at least three times.